T4309

Sigma

 

Thrombopoietin human

recombinant, expressed in NSO cells, lyophilized powder, cell culture tested

This product number has been discontinued but a similar product may be available. Please select the recommended replacement product below (if a direct replacement is available) or use the links to "similar products" to see other possible replacement products.
Synonym:c-MPL ligand, Megakaryocyte colony-stimulating factor, MGDF, TPO
MDL number:MFCD01095335

Description

Analysis NoteThe biological activity is measured in a cell proliferation assay using MO7e cells.
Physical formLyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
Biochem/physiol ActionsThrombopoeitin is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis.1,2,3,4 The mature form of TPO is a highly conserved glycoprotein, showing homology among various mammals. It is produced by liver and kidney cells. TPO stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO), and prolactin (PRL).5,6 Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation.7 Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor).

Properties

recombinantexpressed in NSO cells
assay≥97% (SDS-PAGE)
formlyophilized powder
mol wtglycolysylated recombinant product mol wt 82 kDa by SDS-PAGE
 predicted mol wt 35 kDa (332 amino acids)
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

Related Products

Replaced byT1568, Thrombopoietin human

References

Cited Reference1. Lok, S., and Foster, D., The structure, biology and potential therapeutic applications of recombinant thrombopoietin. Stem Cells 12, 586-598, (1994)
 2. Kato, T., et al., Native thrombopoietin: structure and function. Stem Cells 16, 11-19, (1998)
 3. , Kuter, D., et al., eds. Thrombopoiesis and Thrombopoietins Totowa, N.J , (1997)
 4. Callard, R., and Gearing, A. The Cytokine Facts Book New York , (1994)
 5. Heim, M.H., The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. Receptor Signal Transduction Res. 19, 75-120, (1999)
 6. Skoda, R., Specificity of signaling by hematopoietic cytokine receptors: instructive versus permissive effects. J. Recept. Signal Transduct. Res. 19, 741-772, (1999)
 7. Hou, J., Zhan, H., Expression of active thrombopoietin and identification of its key residues responsible for receptor binding. Cytokine 10, 319, (1998)
referenceAvanzi, G., et al., Selective growth response to IL-3 of a human leukemic cell line with megakaryoblastic features. Br. J. Haematol. 69, 359-366, (1988)